International Journal of Growth Factors and Stem Cells in Dentistry

: 2020  |  Volume : 3  |  Issue : 1  |  Page : 27--34

Socket preservation using demineralized tooth graft: A case series report with histological analysis

Alzaga-Vega Marco Tulio1, Chang-Dong Kang2, Ocampo-Acosta Fabian1, Grace Eun Ah Kim2, Hyung-Gyun Kim3, Dong-Seok Sohn4,  
1 Private Practice, Tijuana, Mexico
2 Department of Dentistry and Oral and Maxillofacial Surgery, School of Medicine, Catholic University of Daegu, Republic of Korea
3 Department of Dentistry and Advanced General Dentistry, Catholic University Hospital of Daegu, Daegu, Republic of Korea
4 Department of Dentistry and Oral and Maxillofacial Surgery, Catholic University Hospital of Daegu, Daegu, Republic of Korea

Correspondence Address:
Dr. Dong-Seok Sohn
Department of Dentistry and Oral and Maxillofacial Surgery, Catholic University Hospital of Daegu, 3056-6 Daemyung 4-Dong, Nam-Gu, Daegu 705-034
Republic of Korea


Purpose: The aim of the study is to histologically evaluate new bone formation in extraction sockets augmented with autologous demineralized dentin bone (ADDB). Materials and Methods: Teeth were extracted from 19 patients and prepared as ADDB and then grafted in 27 extraction socket sites. The graft was covered with membrane made of concentrated growth factors (membrane) and the wounds were closed without periosteal releasing incision (open-membrane technique). Bone biopsy was performed at each implant site after 4 months of healing. Results: Wound healing was uneventful. Histologically, bone reformation was observed in all the augmented sockets, and ADDB showed favorable integration with newly formed bone. Conclusion: Immediate graft after extraction using ADDB is recommended for socket preservation.

How to cite this article:
Tulio AV, Kang CD, Fabian OA, Kim GE, Kim HG, Sohn DS. Socket preservation using demineralized tooth graft: A case series report with histological analysis.Int J Growth Factors Stem Cells Dent 2020;3:27-34

How to cite this URL:
Tulio AV, Kang CD, Fabian OA, Kim GE, Kim HG, Sohn DS. Socket preservation using demineralized tooth graft: A case series report with histological analysis. Int J Growth Factors Stem Cells Dent [serial online] 2020 [cited 2021 Oct 23 ];3:27-34
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Following tooth extraction, volumetric changes in the residual ridge is expected. Difficult tooth extraction procedures may also result in additional bone loss due to surgical trauma.[1] According to Botticelli et al.'s research, buccal bone revealed more severe resorption than lingual bone resorption.[2] Preserving the alveolar ridges at the time of the tooth extraction helps minimize difficulties during subsequent implant placement. Bone graft in extraction socket resulted in lesser change of extraction socket.[3] Long-term esthetic success requires vital alveolar bone to support healthy keratinized tissue and dictate soft tissue profile, which follows the underlying bone contour. Various bone substitutes and several surgical techniques have been employed for socket preservation.[4] Autologous bone graft is considered a gold standard because it shows osteoinductivity, osteoconductivity, and osteogenic capacity.[5] However, due to its drawbacks including morbidity of the second surgical site and prolonged surgical time, various bone substitutes have utilized.[6],[7] These bone substitutes are osteoconductive and mainly function as space makers with volume preservation.[8] The protocol of socket preservation is based on guided bone regeneration using osteoconductive bone substitutes and barrier membrane.[9],[10]

Autologous demineralized dentin bone (ADDB) has been introduced for ridge and sinus augmentation as a new alternative to using autogenous bone graft as it is osteoinductive and osteoconductive.[11],[12],[13] An extracted tooth from the patient was grafted as block or particulate form for socket preservation.[14],[15] Resorbable or nonresorbable barrier membrane is commonly used to prevent soft tissue in growth to the graft site. Autologous fibrin membranes, as barrier membrane, have also revealed favorable bone regeneration with ridge augmentation.[16],[17] Sohn et al. also reported successful guided bone generation using a concentrated growth factor (CGF) barrier membrane with primary wound closure.[18] The aim of this retrospective clinical study is to evaluate augmented socket sites using ADDB and CGF barrier membrane according to histological analysis.

 Materials and Methods

Patients selection

A total of 27 extraction sockets from 19 patients requiring extraction of hopeless teeth were selected. All extraction socket sites were augmented with ADDB and covered with CGF barrier. In three cases, the mandibular 3rd molar was extracted for socket preservation. The average age of this group was 47.8 years old (ranging 19–74). All patients had medical condition with American Society of Anesthesiologists 1 and 2 were selected (healthy patients or patients with controlled systematic diseases). All patients were informed and documented consent obtained for treatment procedure, histological analysis, and restoration procedure [Table 1].{Table 1}

Preparation of autologous demineralized dentin bone

Prophylactic oral antibiotics (Ampliron Dúo, Siegfried Rhein Co., México) were provided to patients, intake starting 1 day before the procedure and continued for 7 days. Chlorhexidine mouthwash (0.2%) was used by patients. Atraumatic extraction was performed under local anesthesia using 2% lidocaine HLC (1:100,000 epinephrine). Preparation of ADDB using extracted teeth was performed chairside. Old restorations, caries, calculus, and soft tissues attached to the extracted teeth were removed with sharp manual instruments and rotary instrument with copious coolant. A surgical mallet was used to particulate the teeth into sizes between 0.8 mm and 1.0 mm. Accelerated demineralization and sterilization of the grafts were achieved using a vacuum-ultrasonic device (VacuaSonic System, CosmoBioMedicare Co., Seoul, Korea) with fifteen minutes of demineralization using 0.6 N hydrochloride and 10 min of sterilization using sterilization reagents (peracetic acid ethanol solution). The demineralized particulate tooth bone was washed and neutralized using phosphate-buffered saline [Figure 1] and [Figure 2].{Figure 1}{Figure 2}

Preparation of sticky autologous demineralized dentin bone and concentrated growth factor barrier membrane

Autologous fibrin glue (AFG) was mixed with ADDB for “sticky ADDB” and CGF barrier was prepared according to Sohn et al.'s protocol.[18] 30–50 cc of patient's venous blood was withdrawn from the patient's forearm, one contained in a noncoated Vacutainer to obtain AFG, and two to four glass-coated Vacutainers. These Vacutainers were centrifuged at 2400–2700 rpm using specific centrifuge (Medifuge, Silfradent srl, Sofia, Italy) for 12 min. ADDB was mixed with AFG – collected from the upper level of the noncoated Vacutainer after centrifugation and allowed for polymerization with ADDB for 5–10 min. The sticky ADDB graft does not separate or migrate due to its interlinked fibrin networks and reduces the healing period. The CGF clot is collected from the middle layer after the glass-coated Vacutainer is centrifuged. The dense clot contains polymerized fibrin and is compressed down flat in a metal box (CGF box) with the cover to be used as a membrane barrier.

Surgical procedure

Any soft tissue or inflammatory tissue in the extraction socket was completely removed by manual curettage. Sticky ADDB was grafted in the socket by gentle compaction and 2 CGF membranes were placed over the graft. Wound was closed with chromic catgut 4-0 suture without periosteal releasing incision (open-membrane technique). After 4 months of healing, full-thickness flap was raised to expose the augmented socket site and 3-mm wide trephine drill was used to collect bone for biopsy and to prepare the osteotomy for implant placement. Implant was placed after consecutive drilling and wound closure using catgut 4-0 suture.

Histological analysis

Collected bone for biopsy was placed in a container of formaldehyde and delivered to the oral pathologist for histological studies. The specimens were fixed for 24 h with neutral buffered formalin, then washed with 0.1 M phosphate buffer solution, and decalcified with 10% formic acid for 5 days. The specimen was embedded in paraffin (Paraplast; Oxford, USA) and sliced coronally into serial sections 5 μm thick. The specimens were stained with hematoxylin and eosin (H and E) and Masson's trichrome (MT) stains, and examined under light microscopy.

Case report 1: Socket preservation and sinus lift lateral approach

A 68-year-old female patient presented with a chief complaint of toothache when biting and mobility Grade III on teeth #13, #14, #15. The patient requested for an implant-supported fixed restoration. Three teeth were removed under local anesthesia using 2% lidocaine (1:100,000 epinephrine) with atraumatic extraction technique also using a Piezotome device to maintain the crestal bone. Manual curettage of the extraction site was performed to remove granulation tissue. The extracted teeth were prepared as particulates and decalcified and sterilized according to the manufacturer's instructions (VacuaSonic®; Cosmobiomedicare, Seoul, Korea). Sticky ADDB was then grafted into the socket with CGF membrane coverage. The wound was closed with chromic catgut 4-0 suture without periosteal releasing incision.

Bone graft site was re-entered after 4 months of healing. Full-thickness flap was elevated to expose the augmented socket and favorable ridge augmentation was seen at the site of the extraction socket. A 3-mm wide trephine drill was used as the initial drill for preparing the osteotomy site for implant placement and provided collection of bone for biopsy.

The specimens were stained with hematoxylin and eosin and cut longitudinally to a thickness of 2–3 microns. Then, magnifications of ×100 were made to determine the rate of bone regeneration. Four months after implant placement and healing, an impression was taken, and three-unit zirconia bridge was delivered for the patient [Figure 3],[Figure 4],[Figure 5],[Figure 6],[Figure 7,[Figure 8].{Figure 3}{Figure 4}{Figure 5}{Figure 6}{Figure 7}{Figure 8}

Case report 2: Socket preservation and sinus lift crestal approach with Lisa technique

A 50-year-old male patient presented with root canal treated teeth #26 and #27 requiring removal due to vertical fractures. The patient requested for implant-supported fixed restoration.

Under local anesthesia using 2% lidocaine (1:100,000 epinephrine), atraumatic extraction of both teeth was performed. Sticky ADDB is grafted to extraction socket [Figure 9] and [Figure 10]. Then, CGF membrane was applied to cover the bone graft. The wound was closed with chromic catgut 4-0 suture without periosteal releasing incision. Subsequent to 4 months of healing, full-thickness flap was elevated to expose the augmented socket and 3-mm wide trephine drill was used as 1st osteotomy drill at implant site to take bone biopsy. The specimens were stained with H and E and MT under light microscopy. Then, magnifications of × 40 and × 100 were made to determine the rate of bone regeneration. After 4 months of healing, screwed retain restoration made from a structure of Pekkton and layering composite as final restoration [Figure 11] and [Figure 12].{Figure 9}{Figure 10}{Figure 11}{Figure 12}

Case report 3: Alveolar three-dimensional ridge reconstruction with autologous tooth block and particulate bone

A 74-year-old patient presented with a fistula on #37. The patient requested for implant-supported fixed restoration. Under local anesthesia using 2% lidocaine (1:100,000 epinephrine), atraumatic extraction was performed. ADDB and demineralzied tooth block was grafted on the defect and CGF membrane was applied to cover the extraction socket on the same day. After 4 months of healing period, the patient returned with exposure of graft, but was unaware of the situation and asymptomatic. A full-thickness flap was elevated to expose the graft and a 3-mm wide trephine drill was used to collect bone before implant placement. The specimen was stained with H and E and MT under light microscopy. Then, magnifications of × 40 and × 100 were made to determine the rate of bone regeneration. After 4 months of healing, the patient came back for open tray impression and zirconia screw-retained restoration was delivered. The cross-sectional image of cone-beam computed tomogram shows stable bone augmentation over the implant [Figure 13], [Figure 14], [Figure 15], [Figure 16].{Figure 13}{Figure 14}{Figure 15}{Figure 16}


Clinical results

From a clinical perspective, all cases healed uneventfully except for one case showing exposure of tooth block graft. Four months after initial graft using ADDB, all socket sites resulted in good bone regeneration for implant placement. Favorable ridge augmentation was achieved for implant engagement with initial stability of more than 25 Ncm.

Histological analysis

ADDB was lightly stained and well differentiated from surrounding tissues in MT stain. Four months after socket preservation procedure, no signs of inflammation were found in the MT stains under light microscopy. Bone reformation was observed in all biopsy samples and newly formed bone showed integration with the ADDB surface. The examined specimen presents fragments of dentin and normal aspect dentinal tubules interspersed with well-vascularized irregular dense fibrous connective tissue. The examined specimens present with mature and vital lamellar bone with vesicular osteocyte lacunae.


Significant changes in bone and soft tissue occur in the alveolar bone after extraction.[19],[20] The overall resorption of horizontal alveolar ridge has been reported as 50%.[21] Prevention of alveolar bone resorption after extraction is important to reduce the amount of bone augmentation that may be required with future implant placement. Socket preservation is a surgical procedure aimed to preserve the alveolar bone after tooth extraction to eliminate or minimize the need for bone augmentation during implant rehabilitations.[22] Socket preservation technique includes the use of bone or bone substitute grafts with or without membranes.[23]

Bone graft materials used in the field of dental and oral surgery can be classified into autografts, allografts, alloplasts, and xenografts.[24] Autografts are highly favored due to its osteoinductivity, osteoconductivity, and osteogenicity.[25] However, there are factors to consider such as limited availability or the need of a secondary donor site, resorption of the graft, and high cost.[26] Other graft materials such as bovine bone, allograft, or synthetic bone have been used as an alternative for sinus augmentation. However, the materials are only osteoconductive and adequate as space makers. To overcome the limitations of autografts and allograft or synthetic bone, researches on ADDBs are drawing much attention.[27] Teeth are composed of inorganic substances such as hydroxy apatite and calcium phosphate, and organic substances such as collagen. The composition of teeth is very similar to the bone,[13] especially the dentin which contains 65% inorganic substances, 35% organic substances, and alveolar bone composed of 65% inorganic substances 35% organic substances.[28] Type-1 collagen and various growth factors such as bone morphogenic proteins (BMP) are contained in dentin. BMPs play a role in differentiating perivascular mesenchymal stem cells into cartilage and bone tissue. Various studies have showed the osteoinductivity of BMP.[29],[30],[31] While Type-1 collagen accounts for nearly 90% of the organic substances, other substances such as noncollagenous proteins (NCP) account for the rest. The Type-1 collagen molecule is a triple-helix and has been proved to show biocompatibility and interfere in bone formation at bone graft recipient sites or dental implant sites. NCPs are known to contribute to bone mineralization by facilitating bone resorption and by inducing bone generation process.[32],[33],[34],[35] Seventy percent of the inorganic substances of dentin are hydroxyapatite, which are low-crystalline apatite. Bones are also composed of low-crystalline apatite.[36] In a study by Xu et al., ADDB could be an excellent alternative to autogenous bone graft, since it has osteoinductivity and osteoconductivity and shows bone regeneration.[37] Favorable clinical results were obtained, and optimal osteoconductivity and osteoinductivity of graft was made when teeth were demineralized for 15 min using 0.6 N hydrochloride.[12],[38] Several researches on histology showed that ADDB resorbed with time and resulted in good bone regeneration or new bone formation.[26],[37],[39],[40],[41] ADDB is not only good in providing scaffolds for bone formation but also highly biocompatible, and when compared with other bone substitutes, ADDB has low antigenicity due to its structural similarity with autologous bone. It would also eliminate any concerns of possible risk of cross-infection that may result from using animals or other human sources.[11],[42] Furthermore, ADDB has osteoinductive properties with high potential for bone remodeling and usually does not require additional grafts such as autologous block bone.[43],[44],[45],[46]

The use of autologous bone or bone graft with membrane enhances to the alveolar healing process using biological factors. Choukron's platelet-rich fibrin and Sacco's CGF membrane are recently developed fibrin membrane with platelet aggregation.[47],[48] CGF membranes have successfully substituted allogenic or alloplastic barrier membranes, with excellent clinical results in both soft and hard tissue healing.[48] Using CGF membranes, successful wound healing at bone augmentation site is shown, even without primary suturing. Furthermore, complications due to early exposure of allogenic or alloplastic membranes may be prevented.[49] Mineral tooth block graft showed an increased risk of dehiscence ranging from 12.96% to 34.38%.[50]

To prevent early exposure of tooth block, microperforations and demineralization process are essential to allow blood infiltration through the tooth graft.[46] In addition, sharp edge of tooth block should be smoothened before placing it in the bony defect. The clinical cases demonstrate how ADDB with CGF membrane can be used for socket preservation resulting in favorable ridge augmentation and less ridge resorption.

According to the histological analysis, significant new bone formation was revealed at 4 months of healing. ADDB showed gradual resorption by osteoclastic activity and volume of tooth graft reduced over time during the bone remodeling process. Furthermore, more mature lamella bone was revealed with time and dense and high connection of tooth bone and newly formed bone was evident.


According to the present study, using histological analysis, it can be concluded that ADDB is a bone graft material with favorable clinical and histological outcomes and should be considered as a noble bone grafts for ridge preservation or augmentation.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.


1Horowitz R, Holtzclaw D, Rosen PS. A review on alveolar ridge preservation following tooth extraction. J Evid Based Dent Pract 2012;12:149-60.
2Botticelli D, Berglundh T, Lindhe J. Hard-tissue alterations following immediate implant placement in extraction sites. J Clin Periodontol 2004;31:820-8.
3Nevins M, Camelo M, de Paoli S, Friedland B, Schenk RK, Parma-Benfenati S, et al. A study of the fate of the buccal wall of extraction sockets of teeth with prominent roots. Int J Periodontics Restorative Dent 2006;26:19-29.
4Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: An update. Injury 2005;36 Suppl 3:S20-7.
5Boyne PJ, James RA. Grafting of the maxillary sinus floor with autogenous marrow and bone. J Oral Surg 1980;38:613-6.
6Younger EM, Chapman MW. Morbidity at bone graft donor sites. J Orthop Trauma 1989;3:192-5.
7van den Bergh JP, ten Bruggenkate CM, Krekeler G, Tuinzing DB. Sinusfloor elevation and grafting with autogenous iliac crest bone. Clin Oral Implants Res 1998;9:429-35.
8Buser D, Martin W, Belser UC. Optimizing esthetics for implant restorations in the anterior maxilla: Anatomic and surgical considerations. Int J Oral Maxillofac Implants 2004;19 Suppl: 43-61.
9Tan WL, Wong TL, Wong MC, Lang NP. A systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans. Clin Oral Implants Res 2012;23 Suppl 5:1-21.
10Chu SJ, Sarnachiaro GO, Hochman MN, Tarnow DP. Subclassification and clinical management of extraction sockets with labial dentoalveolar dehiscence defects. Compend Contin Educ Dent 2015;36:516, 518-20, 522 passim.
11Yeomans JD, Urist MR. Bone induction by decalcified dentine implanted into oral, osseous and muscle tissues. Arch Oral Biol 1967;12:999-1008.
12Huggins CB, Urist MR. Dentin matrix transformation: Rapid induction of alkaline phosphatase and cartilage. Science 1970;167:896-8.
13Kim YK, Lee J, Um IW, Kim KW, Murata M, Akazawa T, et al. Tooth-derived bone graft material. J Korean Assoc Oral Maxillofac Surg 2013;39:103-11.
14Kim YK, Yun PY, Um IW, Lee HJ, Yi YJ, Bae JH, et al. Alveolar ridge preservation of an extraction socket using autogenous tooth bone graft material for implant site development: Prospective case series. J Adv Prosthodont 2014;6:521-7.
15Kim ES. Autogenous fresh demineralized tooth graft prepared at chairside for dental implant. Maxillofac Plast Reconstr Surg 2015;37:8.
16Anitua E, Orive G, Pla R, Roman P, Serrano V, Andía I. The effects of PRGF on bone regeneration and on titanium implant osseointegration in goats: A histologic and histomorphometric study. J Biomed Mater Res A 2009;91:158-65.
17Plachokova AS, Nikolidakis D, Mulder J, Jansen JA, Creugers NH. Effect of platelet-rich plasma on bone regeneration in dentistry: A systematic review. Clin Oral Implants Res 2008;19:539-45.
18Sohn DS, Huang B, Kim J, Park WE, Park CC. Utilization of autologous concentrated growth factors (CGF) enriched bone graft matrix (Sticky Bone) and CGF-enriched fibrin membrane in implant dentistry. J Implant Adv Clin Dent 2015;7:11-29.
19Schropp L, Wenzel A, Kostopoulos L, Karring T. Bone healing and soft tissue contour changes following single-tooth extraction: A clinical and radiographic 12-month prospective study. Int J Periodontics Restorative Dent 2003;23:313-23.
20Pietrokovski J, Massler M. Alveolar ridge resorption following tooth extraction. J Prosthet Dent 1967;17:21-7.
21van der Weijden F, Dell'Acqua F, Slot DE. Alveolar bone dimensional changes of post-extraction sockets in humans: A systematic review. J Clin Periodontol 2009;36:1048-58.
22Annunziata M, Guida L, Nastri L, Piccirillo A, Sommese L, Napoli C. The role of autologous platelet concentrates in alveolar socket preservation: A systematic review. Transfus Med Hemother 2018;45:195-203.
23Trombelli L, Farina R, Marzola A, Bozzi L, Liljenberg B, Lindhe J. Modeling and remodeling of human extraction sockets. J Clin Periodontol 2008;35:630-9.
24Urist MR, Silverman BF, Büring K, Dubuc FL, Rosenberg JM. The bone induction principle. Clin Orthop Relat Res 1967;53:243-83.
25Whitman DH, Berry RL, Green DM. Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 1997;55:1294-9.
26Kim YK, Kim SG, Byeon JH, Lee HJ, Um IU, Lim SC, et al. Development of a novel bone grafting material using autogenous teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:496-503.
27Jeong HR, Hwang JH, Lee JK. Effectiveness of autogenous tooth bone used as a graft material for regeneration of bone in miniature pig. J Korean Assoc Oral Maxillofac Surg 2011;37:375-9.
28Urist MR, Strates BS. Bone morphogenetic protein. J Dent Res 1971;50:1392-406.
29Butler WT, Mikulski A, Urist MR, Bridges G, Uyeno S. Noncollagenous proteins of a rat dentin matrix possessing bone morphogenetic activity. J Dent Res 1977;56:228-32.
30Conover MA, Urist MR. Dentin matrix morphogenetic protein. Paper Presented at: Chemistry and Biology of Mineralized Connective Tissues. Chicago: Northwestern University Dental School; 1981. p. 3-7.
31Hanamura H, Higuchi Y, Nakagawa M, Iwata H, Nogami H, Urist MR. Solubilized bone morphogenetic protein (BMP) from mouse osteosarcoma and rat demineralized bone matrix. Clin Orthop Relat Res 1980;148:281-90.
32Um IW. Extracted tooth: Can it be a bone graft substitute? Adv Dent Oral Health 2017;(1):1-3.
33Bessho K, Tagawa T, Murata M. Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction. J Oral Maxillofac Surg 1990;48:162-9.
34Hoeppner LH, Secreto F, Jensen ED, Li X, Kahler RA, Westendorf JJ. Runx 2 and bone morphogenic protein 2 regulate the expression of an alternative Lef1 transcript during osteoblast maturation. J Cell Physiol 2009;221:480-9.
35Bessho K, Tagawa T, Murata M. Comparison of bone matrix-derived bone morphogenetic proteins from various animals. J Oral Maxillofac Surg 1992;50:496-501.
36Kim GW, Yeo IS, Kim SG, Um IW, Kim YK. Analysis of crystalline structure of autogenous tooth bone graft material: X-Ray diffraction analysis. J Korean Assoc Oral Maxillof Surg 2011;37:225-8.
37Xu X, Sohn DS, Kim HG, Lee SJ, Moon YS. Comparative histomorphometric analysis of maxillary sinus augmentation with deproteinized bovine bone and demineralized particulate human tooth graft: An experimental study in rabbits. Implant Dent 2018;27:324-31.
38Urist MR. Bone histogenesis and morphogenesis in implants of demineralized enamel and dentin. J Oral Surg 1971;29:88-102.
39Masaru Murata TK, Kawakami T, Akazawa T, Tazaki J, Katsutoshi IT, Kusano K, et al. Human acid-insoluble dentin with BMP-2 accelerates boneinduction in subcutaneous and intramuscular tissues. J Ceramic Soc Japan 2010;116:438-41.
40Kim YK. The evaluation of postoperative safety of autogenous teeth bone graft. J Korean Acad Implant Dent 2009;28:29-35.
41Kim YK. Clinical application and classification of bone graft material according to component. J Korea Dent Assoc 2010;48:263-74.
42Carvalho VA, Tosello Dde O, Salgado MA, Gomes MF. Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles. Int J Oral Maxillofac Implants 2004;19:679-86.
43Ritchie HH, Ritchie DG, Wang LH. Six decades of dentinogenesis research. Historical and prospective views on phosphophoryn and dentin sialoprotein. Eur J Oral Sci 1998;106 Suppl 1:211-20.
44Handschin AE, Egermann M, Trentz O, Wanner GA, Kock HJ, Zünd G, et al. Cbfa-1 (Runx-2) and osteocalcin expression by human osteoblasts in heparin osteoporosis in vitro. Clin Appl Thromb Hemost 2006;12:465-72.
45Sohn DS, Moon YS. Histomorphometric study of rabbit's maxillary sinus augmentation with various graft materials. Anat Cell Biol 2018;51:S1-12.
46Moon YS, Sohn DS, Kim G, Park I. Comparative histomorphometric evaluation of bone regeneration with different preparations of xenogeneic tooth block bone. Int J Oral Maxillofac Implants 2019;34:1413-22.
47Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303.
48Rodella LF, Favero G, Boninsegna R, Buffoli B, Labanca M, Scarì G, et al. Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction. Microsc Res Tech 2011;74:772-7.
49Sohn DS, Kim HG. Simplified ridge and extraction socket augmentation using Sohn's poncho technique. J Implant Adv Clin Dent 2018;10:16-36.
50Gharpure AS, Bhatavadekar NB. Clinical efficacy of tooth-bone graft: A systematic review and risk of bias analysis of randomized control trials and observational studies. Implant Dent 2018;27:119-34.